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Judith Rolandelli has had a varied work experience. In 2021, they began working at Expivi as a Project Manager and Sales Executive. In 2020, they were a Business Development Manager Germany at MyPup, My Pick Up Point. From 2017 to 2020, they worked at Fluke Corporation as a Distribution Sales Specialist - Fluke Networks and Customer Service Agent. In 2015, they completed an internship at the Freie Universität Berlin, where they worked on a master thesis project in evolutionary biology. In 2012, they completed an internship at Bioprogramm, where they learned how to investigate the quality of terrestrial and aquatic environments.

Judith Rolandelli completed their Bachelor's Degree in Environmental Sciences from the Università degli Studi di Padova between 2010 and 2013. Judith then went on to earn their Master's Degree in Evolutionary Biology from Alma Mater Studiorum – Università di Bologna between 2013 and 2016.