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Barbora Kalna has worked in a variety of roles since 2007. Barbora began their career as a Translator for the European Parliament in 2007. In 2008, they became a Human Resources Development Specialist at PepsiCo. From 2018 to 2019, they worked as a Human Resources Manager at MPZ MB s.r.o. - průmyslová automatizace, strojírenství. In 2019, they became the Zakladatel of Srdce myslivny, z.s. From 2020 to 2022, they served as a Customer Support/HR Manager at EIN Presswire. Currently, they are a Human Resources Manager at Newsmatics.

Barbora Kalna attended Charles University from 2000 to 2008, where they earned a Master's degree in Social Anthropology. Barbora is currently enrolled at Elio, z.s. and is expected to complete a Psychoanalysis and Psychotherapy program by 2026.