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Nikola Tlustá has a diverse work experience, starting with their role as a Manual Worker at Tesco in 2018. Nikola then worked as a SPA Assistant at Montage Deer Valley in 2019. Following that, Nikola joined Raiffeisenbank Česká republika as an HR Trainee from June 2021 to June 2022. In 2022, they worked as a Junior HR Project and Marketing Specialist at ABSOLVENTI.cz from July to December. In 2023, Nikola held the position of Internal Communication and Development Intern at DODO from February to April. Currently, they are working as a Junior Marketing Specialist at Ecomail.cz.

Nikola Tlustá's education history begins in 2014 when they attended Masarykova obchodní akademie in Rakovník, where they pursued Ekonomické lyceum. Nikola completed their studies there in 2018. In 2018, they enrolled at Prague University of Economics and Business to pursue a Bachelor's degree in Podniková ekonomika a management, which they completed in 2021. During their studies, they also attended a marketing program called "#HolkyzMarketingu" in 2023. Currently, from 2021 to 2024, Nikola is pursuing their Inženýr (Ing.) degree in Management at the Prague University of Economics and Business.