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Anne Kihlgren began their career in 1984 as a Laboratory Engineer at AB Bofors. In 1992, they moved to SKL- National Laboratory of Forensic Science / NFC-National Forensic Centre, where they held the role of Scientist, reporting officer and project leader. In 2000, Anne became the Founder and Advisor in business strategy and product development for Dynamic Code AB, and served as CEO from November 2000 to October 2020.

Anne Kihlgren attended Linköping University from 1988 to 1991, where they earned their Licentiate degree in Biochemistry.