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Timothy Guyon has had a varied work experience, beginning in 2004 as a Medical Imaging Assistant and Transporter at Exempla Lutheran Medical Center. In 2008, they worked as an MRI Technologist Assistant at UNIVERSITY OF COLORADO HOSPITAL ANSCHUTZ INPATIENT PAVILION and a Student Research Assistant at Bristow Cardiology Laboratory Spring 2008. In 2009, they were an HLA Epitope Analyst (Volunteer) at CLINIMMUNE LABS. From 2010-2012, they worked as a Collegiate Medical Volunteer at LAC+USC Medical Center and a Graduate Student Research at Keck School of Medicine of the University of Southern California. In 2012, they began their employment at DxTerity, where they held roles as a Principal Scientist, Staff Scientist, Senior Scientist, Scientist, Senior Research Associate, and Research Associate. In 2018, they moved to RootDx, where they are currently employed as a DxDirect Assay Development Lead.

Timothy Guyon received a Bachelor of Science in Biology from the University of Colorado Denver in 2006-2008. Timothy then attended the University of North Carolina at Chapel Hill from 2004-2005, studying Biology/Biological Sciences, General. In 2011, they obtained a Master of Science in Molecular Microbiology and Immunology from the Keck School of Medicine of the University of Southern California.