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Marie Kalousová began their work experience in 2007 as a Hostess and Promoter for various companies. From 2014 to 2018, they worked at the National Film Archive, where they served as an Editor for the database portal and an External Editor for the Film Overview. In 2015, they worked as a Fundraiser at #‎OSAfilm‬. Marie then joined Czechitas in 2019 as a Workshopist until September 2020. In 2018, they worked at Magistrát města Brna as a Drug Coordinator and as an Assistant to the Released Member of the City Council. Currently, they are working at Dateio as a Data Enhancement specialist starting from August 2022.

Marie Kalousová's education history includes multiple degrees attained from Masaryk University Brno. From 2010 to 2015, they pursued a Bachelor's degree in "Teorie a dějiny filmu a audiovizuální kultury." Afterwards, they continued their studies at the same university and obtained another Bachelor's degree in "Teorie a praxe přípravného řízení trestního" from 2010 to 2014. Later, from 2015 to 2018, they completed their Master's degree in "Management v kultuře" at Masaryk University Brno. Prior to attending university, they attended Jazyková škola Pelikán in 2009-2010, where they studied English and Spanish. No degree was mentioned for this language school.