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Francene Barno has a solid work experience starting from 1991. From 1991 to 2003, they worked at McKolosky Chiropractic as an Insurance/Billing Clerk, where they supervised the billing office and insurance billing and collections. Francene also supervised the office in the absence of the Office Manager and owner. In 2003, they joined Concurrent Technologies Corporation as an Administrative Assistant III and held this position until 2011. Francene then transitioned to the role of Assistant Systems Analyst from 2011 to 2015. In 2015, they were promoted to Associate Systems Analyst/ISSO, and in 2017, they became a System Analyst/ISSO. The end dates for their roles at Concurrent Technologies Corporation are not provided.

Francene Barno obtained an Associate degree in Healthcare Management from Pennsylvania Highlands Community College, which they completed from 2003 to 2006. Francene then pursued further education at Saint Francis University, where they earned a Bachelor of Science degree in Computer Systems Management & Organizational Leadership from 2006 to 2010. Throughout their time at Saint Francis University, their studies focused on VBA and database management/development.