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Tuoyo Louis has a long and varied work experience. In 2014, they were the Chief Operating Officer and Managing Director, as well as the Managing Director, at Zaffre Investments. In 2018, they were the co-founder of Bambü Global Healthcare Advisors, a Member of the Board of Directors at Qcentive, and the Executive Chairman at Yaro. In 2019, they were the co-founder and managing partner at Seae Ventures. In 2020, they were an Observer at Hurdle, and in 2021, they were the Director at Goalsetter, a Board Member at American Council on Exercise (ACE Fitness), an Observer at MD Ally | Public Safety Telehealth, and a Director at Clinify Health.

Tuoyo Louis began their education in 1994, earning a B.A. in Molecular and Cell Biology from the University of California, Berkeley in 1997. Tuoyo then attended Emory University from 2001 to 2004, where they earned a MPH in Health Policy and Management. In 2004, they completed their MBA in Finance from Clark Atlanta University. In 2013, Tuoyo Louis obtained a certification in Leading Innovative Change from the University of California, Berkeley, Haas School of Business.