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Roi Yaacov has worked in various roles since 2001. From 2001 to 2006, they were the Chief Executive Officer of the "Power of Giving" humanitarian organization. From 2006 to 2008, they worked as a Supply Chain Manager for Neopharm Group. From 2008 to 2013, they were the Deputy Head of Mission- Israeli Embassy to Guatemala and Honduras at the Ministry of Foreign Affairs of Israel. From 2013 to 2017, they were the VP Business Development / GUIS CEO at Jethro Group LTD. From 2017 to 2020, they were the Senior Innovation & Business Development Director at the Municipality of Jerusalem. Since 2020, they have been a Business Development Manager at Civan Advanced Technologies and a Family Financial Coach.

Roi Yaacov completed a Graduate degree in Digital Marketing Expert from Mati Jerusalem in 2020, following a Bachelor's degree in Business/Managerial Economics from Ben-Gurion University of the Negev in 2004.