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Nicholas Minutolo has had a varied and successful career in the sciences. Nicholas began their career in 2010 as an Undergraduate Researcher at the University of Connecticut, where they conducted research in the laboratory of Dr. David Knecht and completed a University Scholar Thesis. In 2012, they worked as a Unilever Skin Care Global Design Technician. In 2013, they worked as a Formulation Technician at Pfizer and a Research Associate I, Molecular Sciences at Alexion Pharmaceuticals, Inc. From 2014 to 2020, they were a PhD Student - Pharmacology and Translational Therapeutics at the University of Pennsylvania, where they conducted dissertation work in the laboratory of Dr. Daniel J. Poewll, Jr. Since 2020, they have been a Senior Scientist, Scientist II, and Scientist at Carisma Therapeutics.

Nicholas Minutolo received their Bachelor of Science in Cell/Cellular and Molecular Biology from the University of Connecticut in 2012. Nicholas then went on to receive a Master's degree in Developmental and Cell Biology from the same university in 2012. Finally, they earned a Doctor of Philosophy in Pharmacology from the University of Pennsylvania School of Medicine in 2019.