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Nina Spitz began their work experience in 1996 as a Freelancer for the Foundation "Schweizer Jugend forscht", where they worked until 2003. In 2005, they joined Prosecus GmbH as a Product Manager until 2006. Nina then moved on to Biosynth Carbosynth in 2006, where they served as the Head of Quality Assurance. No specific end dates were provided for any of these roles.

Nina Spitz obtained their Dr. sc. nat. ETH degree in organic chemistry from ETH Zürich, where they also completed their diploma in chemistry with a focus on organic and analytical chemistry. Additionally, they attended Ecole Nationale Supérieure de Chimie in Paris for a brief period.