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Kristal Pena started their work experience in 2009 as a Medical Assistant at Gabriel Gustin, M.D., where they improved their skills in phlebotomy and EKG. Kristal then worked as a Medical Assistant at Bae Medical P.C., where they learned efficiency and time management skills, performing various medical tasks such as EKGs, PFTs, and blood work. Later, they joined Doctors At Trump Place as a Medical Assistant, responsible for greeting patients, recording vital signs, and conducting diagnostic exams. After that, they worked as a Certified Medical Assistant at Neway Fertility for a short period. Kristal then joined Reproductive Medicine Associates of New York as a Clinical Medical Assistant, gaining experience in reproductive medicine. In 2016, they became a Certified Medical Assistant at New York-Presbyterian/Queens. Most recently, they started working as a Medical Aesthetician at Bella Body Medical Spa in 2021.

Kristal Pena has a diverse education history. Kristal attended Freedom High School from 2006 to 2007 and earned their High School Diploma. Later, in 2008 and 2009, Kristal pursued further education at ASA College, where they acquired an Associate's Degree in Allied Health and Medical Assisting Services. Additionally, during the same period, they also obtained an Associate's Degree in Phlebotomy Technician/Phlebotomist from ASA College. Afterward, Kristal attended Hunter College from 2015 to 2018, where they earned a Bachelor's Degree in Registered Nursing/Registered Nurse. Most recently, from 2020 to 2021, they attended the Long Island Beauty School-Hempstead to specialize in Aesthetician/Esthetician and Skin Care Specialist.