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Niklaus Röthlin has a diverse work experience in various companies. Niklaus started their career as a Laboratory Technician at Blaser Swisslube in 2010 and worked there until February 2013. Niklaus then moved on to Crucell, where they served as a Laboratory Technician from February 2013 to July 2014. Following that, they joined PaxVax, Inc. as a LIMS Administrator/Sr. Lab Technician and worked there for five years, from August 2014 to November 2019. After PaxVax, they joined Emergent BioSolutions as a Sr. LIMS Administrator and later transitioned to the role of Specialist Quality IT Systems. Niklaus worked at Emergent BioSolutions from November 2019 until May 2023. Currently, Niklaus Röthlin is employed at Bavarian Nordic as a Specialist Quality IT Systems, starting in 2023.

Niklaus Röthlin completed their education in the field of chemistry. From 2006 to 2009, they attended Amt für Umweltschutz Bern, Stadtlabor, where they earned a Chemielaborant, EFZ degree. During the same period, they also attended gibb Berufsfachschule Bern, although the specific degree or field of study for this institution is not mentioned. Additionally, Niklaus Röthlin obtained a certification in "Einführung in die HPLC von SCS" from the Division Analytische Wissenschaft der schweizerischen Chemischen Gesellschaft, although the exact year and month of accomplishment are not provided.