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Pat O'Sullivan has an extensive work history in the pharmaceutical industry. From 1994 to 1999, O'Sullivan worked as a Manufacturing Manager at SmithKline Beecham. In 1999, they joined Stryker Instruments as an Operations Manager until 2001. From 2001 to 2022, O'Sullivan held various roles at Sanofi, including Vice President and General Manager of a biotechnology manufacturing plant, Vice President and General Manager of Genzyme Waterford, and Vice President Supply Chain for Sanofi Genzyme and Biologics Industrial Platform. Currently, O'Sullivan is working as the Chief Technical Operations Officer at Amicus Therapeutics.

From 1986 to 1990, Pat O'Sullivan attended Munster Technological University, where they pursued a degree in Chemical Engineering. No specific field of study was mentioned.