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Jon Retzlaff's work experience includes serving as the Chief Policy Officer & Vice President of Science Policy and Government Affairs at the American Association for Cancer Research since 2010. Prior to that, they worked as a Lobbyist for non-profits at Lewis-Burke Associates LLC from 2008 to 2010. From 2004 to 2008, they served as the Director of Legislative Affairs at the Federation of American Societies for Experimental Biology. Before that, they worked as the Executive Officer at the National Library of Medicine at the National Institutes of Health from 2002 to 2004. Jon also had a role as a Staff member at the United States Senate Committee on Appropriations from 2000 to 2001.

Jon Retzlaff has a strong education background, with degrees from prestigious institutions. Jon completed their Bachelor of Science (BS) in the field of study of Carlson School of Management at the University of Minnesota from 1985 to 1989. Following this, they pursued a Master of Public Administration (MPA) from Indiana University Bloomington, where they studied from 1991 to 1993. Later, in 2001 and 2002, Jon attended the MIT Sloan School of Management, specifically the Sloan Fellows Program, where they obtained a Master of Business Administration (MBA).