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Dawn James has a range of work experience in the field of scientific research. Dawn began their career as a Teaching Assistant at California State University, Fresno in 2018, where they later transitioned to the role of Research Assistant. In this position, they collaborated with Quest Pharmatech on the expression and purification of recombinant proteins. Dawn utilized techniques such as SDS-PAGE and Western blotting for protein analysis. Dawn then joined Valley Children's Healthcare in 2019 as a Research Assistant, where they performed validation studies on nucleic acid detection assays and maintained regulatory paperwork for the lab. Dawn used PCR for diagnosing various bacteria and viruses. Dawn's most recent position is as a Scientist at Alphazyme LLC, starting in March 2020.

Dawn James obtained a Master's degree in Biotechnology from California State University, Fresno. Dawn pursued this degree from 2018 to 2020. Prior to their master's program, Dawn completed their Bachelor's degree in Biochemistry, Chemistry, and Zoology at Panjab University. Dawn attended Panjab University from 2015 to 2017.