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Jonathan Rothberg has a long history of founding and chairing various companies in the technology and biotechnology fields. Jonathan began their career in 2004 when they founded RainDance Technologies, where they served as Chairman and Founding CEO. In 2007, they founded Ion Torrent by Life Technologies and served as Founder, Chairman, CTO and CEO. In 2011, they founded Butterfly Network, Inc., where they served as Founder, Chairman, and Product Architect. In 2013, they founded AI Therapeutics and served as Founder and Chairman. In 2014, they founded 4Catalyzer, Hyperfine, and Identifeye Health, where they served as Founder and Chairman for each. In 2015, they founded Quantum-Si and began serving as Founder and Chairman, as well as becoming an Adjunct Professor of Genetics at Yale School of Medicine. In 2018, they founded Detect and served as Founder and Chairman.

Jonathan Rothberg attended Carnegie Mellon University from 1981 to 1985, where they earned a Bachelor of Science in Chemical Engineering. Jonathan then attended Yale University, where they earned a Doctor of Philosophy in Molecular Biology.