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Anne DiFiore has a diverse work experience spanning over two decades. Anne began their career as a Grain Merchant at Cargill in August 1998 and worked there until April 2001. Following that, they joined Wilbur-Ellis Company as a Senior Marketing Manager in July 2001 and stayed with the company until May 2010. In June 2010, Anne became the Owner of TerraMar Ingredients LLC, where they marketed niche proteins and oils to domestic and international aquaculture and pet food companies. Anne handled all aspects of the business, including logistics and product distribution, until October 2020. In October 2020, Anne joined LinkOne Marine Solutions as Vice President of Strategic Sales. Their most recent role is as the Vice President of 3D Marine Solutions at 3D Corporate Solutions, starting in October 2021.

Anne DiFiore's education history includes studying Spanish Language and Literature at the Centro de Lenguas Modernas in Granada, Spain from 1996 to 1997. Anne then pursued a Bachelor's Degree in International Business and Spanish at the University of St. Thomas from 1993 to 1996. Anne also has a background in Economics and Spanish from Lawrence University, although the specific dates of study are not provided. Anne attended Virginia High School as well.