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Anna Duke has a diverse work experience spanning over three decades. Anna began their career in 1997 at Suffolk County Council as an Information Service Officer. Anna then moved on to Customer Service Direct, where they assumed the role of Information Services Manager from 2004 to 2007. In 2012, Anna joined New York-New York Hotel & Casino as a Recreations Services Supervisor, eventually getting promoted to Recreation Services Manager until 2018. In the same year, they joined Zenoti as a Project Manager and later became the Director of Key Accounts in November 2020.

Anna Duke attended Carter High School from 1988 to 1989. There is no information provided regarding the degree or field of study they pursued during this time. Anna then enrolled in the University of Suffolk from 2000 to 2002, where they obtained a Higher National Diploma (HND) in Information Services Software Engineering. Anna later attended Arkansas State University-Beebe from 2010 to 2012, earning two Associate's Degrees. The first degree was in Health Services/Allied Health/Health Sciences, General, while the second degree was in General Studies.