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Colleen Wheeler has worked for multiple companies in the healthcare industry, starting in 2007 as a Public Health Analyst for the US Department of Health and Human Services. In 2008, they became an Epidemiologist and later a Research and Evaluation Manager for the Arizona Department of Health Services, where they worked until 2010. In 2009, they founded Wheeler Medical LLC and held the position of VP of Quality & Compliance.

Colleen Wheeler received their Bachelor of Science degree in Economics from the W.P. Carey School of Business at Arizona State University in 1998. Colleen then pursued a Master of Public Health degree in International Health at The Johns Hopkins University from 2006 to 2009. Prior to that, from 2003 to 2007, they also completed a Master of Health Services Management degree with a specialization in Managerial Epidemiology at Arizona State University.