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Karen Tuke's work experience includes various roles in different companies. Karen currently works as an Exam Invigilator at Outwood Hasland Hall Academy and Outwood Academy Portland. Previously, they worked as a Community Host at Vivify, where they welcomed community clubs and sports to the school's sports facilities. Before that, they worked as a Dispensing Optician at Specsavers and Eyeye. Karen also had a role as a Retail Manager/Dispensing Optician at Specsavers. In the past, they served as a Regional Mission and Purpose Champion at Boots Opticians, supporting the Nottingham and South Yorkshire region through changes in the company's customer experience.

Karen Tuke attended Anglia Ruskin University, where they pursued a degree in Ophthalmic Dispensing, with a focus on Optics/Optical Sciences. The specific start and end years of their education are not provided.