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Astrid Coolen has worked in a variety of roles since 2010. Astrid began their career as an intern at Leiden University Medical Center in 2010, followed by an internship at RIVM in 2011. In 2012, they joined Intravacc as a Research Technician Molecular Virology, where they also worked as a Research Technician USP, performing cell and virus cultures from small scale to 100 L. In 2022, they moved to VectorY, where they have served as a Senior Technician Vector Generation and Technician Vector Generation.

Astrid Coolen attended Fontys Hogeschool Eindhoven from 2008 to 2012.