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Tarsila da Cruz's work experience includes serving as a Simulation Project Lead and Simulation Specialist/Outreach Program Lead at Sunnybrook since July 2021 and May 2022 respectively. Prior to that, they worked as a Patient Flow Coordinator at University Health Network from November 2020 to July 2021. Tarsila also worked as a Training Manager at HSE Elements Inc from June 2019 to April 2020, and as an HSE Consultant at Executive HSE Group from June 2018 to April 2020. Tarsila's earlier experience includes roles such as Training Coordinator – Simulation Centre at Hospital Israelita Albert Einstein from March 2015 to September 2016, and Quality Nurse Improvement Internship at Hospital Israelita Albert Einstein from March 2013 to December 2014. Tarsila also served as a Class Representative at Centro Universitário São Camilo from February 2011 to December 2014, and as a Nursing Laboratory Responsible from March 2012 to present.

Tarsila da Cruz's education history includes a post-graduation degree in Occupational Health and Safety Management from Lambton College (2017-2019). Tarsila also holds a Bachelor's degree in Registered Nursing from Centro Universitário São Camilo (2011-2014). Additionally, they have obtained various certifications, including a Bachelor's degree in Nursing from Centro Universitário São Camilo, and certifications in Educator Development, Code White, OSLT Communication in the Healthcare Sector, and others.