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Jen Lee has a diverse work experience. Jen began their career as a Volunteer at the American Red Cross in 2001, holding various roles such as Club President, Vice President, Fundraising Director, and Volunteer until 2008. From 2007 to 2009, they worked as a Docent at the International Museum of Surgical Science. In 2009, they became an Immigration Paralegal at the Law Office of Douglas Bristol, where they worked until 2010. From 2012 to 2014, they worked at 3Red, first as an Accounting/Benefits employee and then as an Office Manager. During this time, they also volunteered as a Service Unit Manager and Troop Leader at the Girl Scouts of Greater Chicago & Northwest Indiana from 2012 to 2019. Currently, they are a Senior Manager in HR Operations at Riskonnect, Inc., a position they have held since 2016.

From 2004 to 2008, Jen Lee attended the University of Chicago, where they pursued a Bachelor of Arts (BA) degree. Their field of study was focused on History, Philosophy, and Social Studies of Science and Medicine.