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Karen Derbyshire has a work history that spans over two decades. Karen started their career in 1998 as a Nursery Manager at The Mill Day Nursery. In 1999, they moved to YMCA Training where they worked as a Location Manager for fifteen years. Karen then joined Realise in 2015 as the Head of Sector Early Years, a role they held until 2023. Later in 2023, they took on the position of Operations Director Early Years at Realise.

Karen Derbyshire attended John Summers High School from 1989 to 1994. However, no information is provided regarding the degree earned or field of study pursued during this time.