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Orissa Agnihotri has a diverse work experience in the food and healthcare industries. In 2008, they began their career as a Session Assistant at Memorial Sloan-Kettering Cancer Center, where they checked in patients and acted as a liaison between patients, nurses, and physicians. In 2010, they began an internship at OHSU, where they studied the immunology and microbiology of Mycobacterium tuberculosis. In 2011, they returned to Memorial Sloan-Kettering as a Research Study Assistant, where they were responsible for database data entry and quality compliance of clinical research. In 2012, they began working at Sullivan Street Bakery as a Retail Sales Associate, providing customer service and assisting in wholesale deliveries. Later that same year, they began working at Raaka Chocolate, first as a Sr. Sales & Trade Marketing Manager and then as Director of Operations. In their role as Director of Operations, they were responsible for human resources, payroll and benefits, tax pay, insurance compliance, bookkeeping, bank account reconciliation, accounts payable and accounts receivable. Orissa also managed Raaka's customer service and spearheaded business development projects.

Orissa Agnihotri attended Reed College from 2007 to 2011, where they obtained a Bachelor of Arts in Biology. During this time, they also attended Pondicherry University in 2009, although no degree or field of study was specified.