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Alison Bartkowski has a diverse work experience spanning various roles and positions. Alison is currently employed at PSignite as a Senior Business Analyst. Prior to this, they worked as an Associate Researcher at the Icahn School of Medicine at Mount Sinai from July 2020 to October 2022. Before that, they held a role as a Researcher at The Johns Hopkins University, specifically at Dr. Jordan Green's lab, from September 2016 to May 2019. Alison also gained experience as a Lab Assistant at PHYSICAL OPTICS CORPORATION from June 2015 to July 2017.

Alison Bartkowski completed their education in the field of Bioengineering and Biomedical Engineering. From 2014 to 2018, they attended Johns Hopkins Whiting School of Engineering and earned a Bachelor of Science degree. Following their undergraduate studies, Alison pursued further education at the same institution and obtained a Master of Science degree from Johns Hopkins Whiting School of Engineering in 2019.