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Madeline Dukes began their professional career in 2004 as an Intern at High Purity Standards. In 2005, they began working as a Graduate Student at Vanderbilt University. In 2011, they joined Protochips as an Applications Scientist and eventually became the Applications Team Lead.

Madeline Dukes received their Bachelor of Science in Chemistry from Charleston Southern University between 2001 and 2005. Madeline then went on to complete their Doctor of Philosophy in Chemistry at Vanderbilt University between 2005 and 2011.