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Emma O'Kane has worked in various roles in the past five years. In 2021, they became the Account Manager & Delivery Lead for Mental Health at Oxehealth. In 2020, they were the Client Success Manager at Pacenet. From 2018 to 2020, they were an Account Manager, Senior Account Executive, and Account Executive at woolleypau. In 2017, they were a Laboratory Research Assistant at the National Children's Research Centre, where they focused on the pathology behind Age-related Macular Degeneration (AMD). In 2016, they were a Laboratory Research Assistant at the University of Oxford's Peter Medawar Building for Pathogen Research, where they conducted research on Hepatitis C.

Emma O'Kane received their Leaving Certificate from St. Vincent's Secondary School in 2013. Emma then attended Trinity College Dublin, graduating in 2017 with a BSc in Human Health and Disease. In 2018, they completed their MSc in Public Health from the London School of Hygiene and Tropical Medicine, University of London.