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Roi Zucker's work experience begins in 2009 at Reut Medical Center, where they worked as a Physiotherapist until 2013. Following this, they joined Roi zucker physiotherapy from 2012 to 2021. From 2013 to 2021, Roi worked at the Israeli social security as a Medical files reviewer. Currently, they are employed at Olala Homes as a Business Development Specialist, starting in 2022.

Roi Zucker attended the University of Haifa from 2005 to 2009, where they pursued a bachelor's degree in physiotherapy. Their field of study throughout their time at the university was focused on physiotherapy.