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Mark Johnson has a diverse work experience, primarily in the non-profit sector. mark served as the Director of Development at Meridian Behavioral Healthcare, Inc. mark then moved on to become the Chief Operations Officer at both the YMCA of North Central Florida and the Arnold Palmer Hospital, Phillips Center for Children & Families. mark also held the role of Vice President of Community Initiatives at the United Way of North Central Florida.

Mark Johnson earned a Bachelor's degree in Education from the University of Florida, which they attended from 1980 to 1984. Following this, they pursued a Master's degree in Education from the same university, completing it in 1985. Johnson continued their academic journey by enrolling in the University of Central Florida, where they obtained a Doctor of Philosophy (Ph.D.) degree in Public Health between 2001 and 2005.