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Daryl Hoelke has worked in a variety of roles since 1977. Daryl began their career with the Canadian Forestry Service, where they held the roles of Tree Physiology and Plasticity and Forest Tree Genetics from 1977 to 1994. Daryl then moved to the Union of Environment Workers, where they served as Executive Assistant to the President from 1994 to 2017. In 2017, they joined the Union of Health and Environment Workers as Executive Assistant - Internal Affairs. Most recently, they have been working as an Online Marketing Specialist for MaxSold since 2020.

Daryl Hoelke attended Algonquin College from 1972 to 1974, where they earned a Forestry Technician degree. Daryl then continued their studies at the University of Florida, focusing on Tree physiology. Daryl then moved on to the University of Guelph, though no further details are provided.