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Bosco Lui began their work experience in 2010 as a Staff Otolaryngologist - Head & Neck Surgeon at Markham Stouffville Hospital. Bosco also served as the Division Head of Otolaryngology - Head & Neck Surgery from 2021 to 2023. In the same year, they became the Medical Director of Markham Ear, Nose & Throat Clinic. In 2011, they joined the University of Toronto as a Lecturer in the Department of Otolaryngology - Head & Neck Surgery.

Bosco Lui completed their Bachelor of Science degree in Honours with a focus on Biological Sciences and Psychology at the University of Toronto. Bosco then pursued their Master of Science in Auditory Neurophysiology, also at the University of Toronto. After that, they attended McMaster University, where they obtained their Doctor of Medicine (MD) degree. Bosco furthered their education at McMaster University by completing a residency in Otolaryngology - Head & Neck Surgery, earning the FRCSC qualification.