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James Wilkinson has a diverse work experience spanning over several companies and roles. James started their professional career as an associate at Johnson and Bussey, PA in 1990 and worked there until 1997. From 1997 to 1998, they served as the managing partner at Brown & Wilkinson, PA. James then became the managing partner at Wheeler & Wilkinson, LLP from 1999 to 2001. In 2002, they joined Dean, Ringers, Morgan and Lawton, PA as a shareholder, where they stayed until 2015. During this time, they also worked as an adjunct law professor at Hamline University from 2010 to 2015. From 2015 to 2017, they held the position of Senior Vice President and General Counsel at Triad Isotopes, Inc. Currently, they hold the role of Vice President Legal at Jubilant Radiopharma, starting in September 2017.

James Wilkinson earned their High School Diploma from New Canaan High School in 1981. James then pursued a Bachelor of Science degree in Political Science from the University of Iowa, which they completed in 1985. James furthered their studies at Mitchell Hamline School of Law, where they obtained a Juris Doctor degree in Law, graduating in 1989.