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David Ben-Ami has a diverse work experience spanning multiple companies and roles. In 2016, they joined SBI JI Innovation Advisory Ltd. as a General Partner. The following year, in 2017, they co-founded and became a Director for Joy Ventures. David also served as a Managing Partner at Corundum Open Innovation from 2016 onwards. In 2017, they took on the role of Director at both ImmunoBrain Checkpoint Inc. and Novolog. Additionally, they became a Director at Chemomab in 2017, but left in 2021. From 2018 to 2020, they served as a Director at Quark Pharmaceuticals Inc. Earlier in their career, they held the position of Director at VVT Med Ltd. from 2008 to 2019. David was a Director at Entera Bio Ltd. from 2014 to 2018, and a Director at Macrocure from 2008 to 2017.

David Ben-Ami attended Tel Aviv University from 1982 to 1986, where they pursued a Bachelor's degree in Economics and Business Administration. Subsequently, from 1986 to 1987, they pursued a Master's degree in Marketing and Management Information Systems (MIS) at Tel Aviv University.