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Anitha Karthikeyan's work experience begins in 2001 at Anusha Engineering Industries, where they served as a Finance and Operations Manager until September 2015. In 2017, they joined Indsafri as a Director and later became the Chief Operating Officer in April 2022.

Anitha Karthikeyan has pursued higher education at Madurai Kamaraj University, where they obtained a Master of Business Administration (MBA) degree with a specialization in Finance. Prior to that, they completed their Bachelor of Commerce (B.Com) degree at the University of Madras. However, the specific duration of their studies at both universities is not provided.