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Ana Santiso has a diverse work experience spanning several industries. Ana started their career at CBS Inc as a product merchandising coordinator from 1987 to 1991. Ana then worked at Physician's Management as an Account Executive from 1991 to 1994. In 2009, they joined Premier Healthcare Resource as a Sales and Marketing Manager until 2010. Ana then moved on to UBM Medica US, where they held various positions including Associate Publisher for Medical Economics Magazine starting in 2010 and Publisher from March 2018 to May 2019. Currently, they are working at Healthline Media as the Director of Advertising Sales, a position they have held since May 2019.

Ana Santiso attended Boston College from 1982 to 1986, earning a Bachelor of Arts degree in English and Marketing.