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Ho-Le Thao Phuong's work experience includes a role as a Visiting Researcher at the Garvan Institute of Medical Research starting in April 2016. Prior to that, they worked as a Project Developer for i2i-Technologies from November 2010 to February 2012. Their earlier experience includes a role as a Database Developer at Viettel Technologies from June 2009 to December 2009.

Ho-Le Thao Phuong began their education at Hatinh High School for the Gifted from 2001 to 2004. Ho-Le then attended the Post and Telecommunications Institute of Technology from 2004 to 2009, earning a Bachelor of Engineering in Information Technology with a GPA of 8.15 out of 10. Additionally, they pursued a Master of Computer Science (Management) at The University of Queensland from 2010 to 2012, achieving a GPA of 6.25 out of 7. Finally, they completed their education by obtaining a Doctor of Philosophy (PhD) in Biomedical/Medical Engineering from the University of Technology, Sydney from 2014 to 2019.