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Justine Lee has a diverse work experience spanning various organizations. Justine began their career at Ernst & Young in 2000 as a Senior Auditor, where they stayed until 2003. From 2003 to 2005, they worked as a Senior in Corporate Finance, focusing on Valuation & Business Modelling. Following that, from 2005 to 2010, they worked as a Manager in Transaction Advisory Services. Justine then took on the role of Assistant Director in Quality & Risk Management from 2010 to 2012.

In 2012, Justine joined SPRING Singapore as the Head of Internal Audit, a position they held until 2015. Since 2015, they have been working at Enterprise Singapore. Justine started as the Deputy General Manager of SEEDS Capital, where they stayed until 2021. In April 2021, they took on their current position as the Deputy Director of Enterprise Leadership.

Justine Lee began their education in 1995 at Raffles Junior College, where they completed their A-level studies in 1996. Following this, they attended Nanyang Technological University in Singapore from 1997 to 2000. During their time at the university, Justine pursued a Bachelor's degree in Accountancy and specialized in the field of Accountancy.