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Charalambous Avgui has extensive work experience in various roles and industries. Charalambous started their career as a Postdoctoral Fellow at the University of Connecticut in 1991. Charalambous then moved on to the University of Michigan where they worked as a Postdoctoral Fellow from 1992 to 1994, and later as a Research Investigator in 1994. In 1995, they joined Elpen Pharmaceutical Co. Inc. as a Counselor to the Chairman and CEO. Charalambous also served as the Business Development Director, Respiratory at the same company.

Charalambous Avgui completed their Bachelor's degree in Pharmacy from Aristotle University of Thessaloniki (AUTH) in an unknown year. Charalambous then pursued further studies and obtained another Bachelor's degree in Pharmacy from the same institution. Later, from 1981 to 1985, they attended the School of Pharmacy at Aristotle University of Thessaloniki, where they earned their first Bachelor's degree. Finally, from 1985 to 1991, Charalambous Avgui attended the University of Connecticut, where they completed their Ph.D. in Medicinal Chemistry.