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Kelly de Ligt has had a diverse work experience in various research roles and leadership positions. Kelly started their career in 2014 as a Research Assistant at IKNL, where they later became a Junior Researcher and then a Researcher. In 2016, they joined the University of Twente as a PhD candidate. In 2020, Kelly worked as a Postdoctoral Researcher and Project Lead at Antoni van Leeuwenhoek. Kelly also became a Redactielid at NVPO in 2021. In 2022, they worked as a Visiting Fellow at the Medizinische Universität Innsbruck.

Kelly de Ligt began their education in 2003 at Liemers College, where they completed their VWO degree in 2010. Following this, they enrolled at the University of Twente and pursued a Bachelor of Science (BSc) degree in Gezondheidswetenschappen, which they completed in 2013. Continuing their education at the same university, Kelly obtained a Master of Science (MSc) degree in Health Sciences in 2014.