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Carmine J. Vacchio, Jr. began their work experience in 2008 as a Secondary Education Teacher at Hauppauge Public Schools, where they taught until 2010. Carmine J. then joined KARL STORZ Endoscopy-America, Inc. in 2010 as an On-Site Endoscopic Specialist, responsible for educating medical staff on instrument use and coordinating surgeon trials. From 2011 to 2013, they took on the role of Sales Professional at KARL STORZ, selling minimally invasive products to hospitals and surgery centers. Starting in 2013, Carmine worked as a District Sales Manager at Cook Medical, focusing on selling speciality urology disposables and equipment to hospitals and physicians. Currently, they hold the position of Regional Account Manager at Dornier MedTech, a role they have held since 2016.

Carmine J. Vacchio, Jr. pursued their education at St. Joseph's University in New York. From 2002 to 2006, they completed their Bachelor of Arts (B.A.) degree in Secondary Education and Teaching. Alongside their formal education, Carmine has obtained additional certifications to enhance their skills. In August 2020, they completed the "Ken Blanchard on Servant Leadership" certification from LinkedIn. Carmine also obtained the "Subtle Shifts in Thinking for Tremendous Resilience" certification from LinkedIn in the same month and year.