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Chloe O'Kane has a varied work experience, beginning with a role as a Shop Assistant at Williamsons Chemist, Finaghy in 2015. In 2017, they took on a Community Health Support Worker role at Early Years - the organisation for young children, in addition to a Family Health Initiative Placement Student position. In 2019, they were a Summer Intern at Queen's University Belfast's Institute of Global Food Security, assisting in the planning, facilitating and evaluating of a pilot cookery intervention. In 2020, Chloe O'Kane began working as an Assistant Project Manager and Graduate Intern at Belfast Health and Social Care Trust, and in 2022 they took on a Project Manager role at Datactics.

Chloe O'Kane completed a Bachelor of Science in Food Quality, Safety and Nutrition with Professional Studies from Queen's University Belfast from 2015 to 2019. Chloe then pursued a Master of Public Health from Queen's University Belfast from 2019 to 2020. Chloe also holds certifications from LinkedIn, ILX Group, CIEH LIMITED, and the Food Standards Agency.