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Kylie Ortego has a diverse work experience in the healthcare field. Kylie started their career in 2002 as a Respiratory Therapist at Our Lady of the Lake Regional Medical Center, where they worked until 2003. From 2003 to 2012, they served as a Registered Respiratory Therapist at Woman's Hospital. In 2019, they joined Cytovale as a Clinical Research Associate and later transitioned to the role of Research and Applications Specialist. In this role, they continue to contribute their expertise and skills in the field.

Kylie Ortego began their education at Louisiana State University in 1994 and completed their Bachelor of Science degree in Industrial and Organizational Psychology in 1999. Following their undergraduate studies, they attended Our Lady of the Lake College from 2001 to 2002, where they earned an Associate of Science degree in Respiratory Care Therapy/Therapist.