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Dr. Motlana holds an MBBch from the University of the Witwatersrand and a MMed in Psychiatry from the University of Pretoria. Dr. Motlana was Clinical Head at Tara Hospital, one of the foremost psychiatric institutions in the country before leaving medicine to pursue a second career in business. She retains the position of an Honorary Consultant at the Outpatient Eating Disorder Unit at Tara Hospital and is involved in research at the University of the Witwatersrand. She is a member of the Hospital Board at Tara Hospital.​

She is the founding director of Psychiatry M Powered, a PBO that seeks to counter the stigmatisation of mental illness through psycho-education. This work has led her to make regular appearances on television, radio and print media. She is also a founding director of the Dr. Nthato Motlana Foundation. Dr. Motlana was appointed to the Clinix Health Group board in 2008 and she also sits on the boards of several companies and organisations.