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Ralph J. de Wit has a diverse and extensive work experience in the medical field. Ralph J. started their career as a medical doctor at the Royal Netherlands Army in 1991 and worked there until 1992. Ralph J. then worked as a resident in the Department of Surgery at Twenteborg ziekenhuis from 1992 to 1994. After that, they served as a surgical resident assigned as Transplant Coordinator at Maastricht UMC+ from 1994 to 1995. Ralph J. then moved to Jeroen Bosch Ziekenhuis, where they worked as a surgical resident from 1995 to 1998. During this time, they also completed a traineeship in intensive care and trauma surgery at UMC Utrecht from 1998 to 2002. In 2002, they joined Medisch Spectrum Twente as a trauma surgeon and (surgical) intensive care specialist. Ralph J. held multiple roles at Medisch Spectrum Twente, including being a member of the Hospital Medical Board and the medical director of the Intensive Care Department. In 2014, they became the Medical Director of Level 1 Trauma Centre and Acute Medical Care for the Region and Cross Border Area at Acute Zorg Euregio. In 2019, they became the Vice President of the Netherlands for CIOMR - Interallied Confederation of Medical Reserve Officers. Additionally, throughout their career, they served as a lieutenant colonel in the Royal Netherlands Army as a military trauma surgeon and worked at the Netherlands Ministry of Defence; Institute for Defence and Partnership Hospitals.

Ralph J. de Wit completed a Certificate Program in Healthcare Management from Nyenrode Business University in 2018. Prior to that, they attended Radboud University from 1983 to 1990, where they studied Geneeskunde. Ralph J. also attended Jacobus College Enschede from 1977 to 1983, where they obtained their Gymnasium degree.