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Lottie Jose's work experience began with a research internship at The University of Huddersfield in 2018. In 2017, Lottie worked as bar staff at St Austell Brewery until 2020. From 2019 to 2021, Lottie served as a Scientist I at Horizon Discovery. Lottie then became a Technical Assistant at bit.bio in 2021 and later transitioned to a Research Assistant role starting in August 2022.

Lottie Jose earned a Bachelor's degree in Biology from Edge Hill University, where they studied from 2016 to 2018. Lottie then went on to pursue a Master's degree in Stem Cell Technology and Regenerative Medicine at the University of Nottingham from 2018 to 2019.