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Denise Harshbarger has a diverse work experience spanning over several industries. Denise started their career as a Medical Technologist at Parkview Health, where they worked from 1996 to 2010. Denise then transitioned to the nonprofit sector, serving as the Office Coordinator at World Relief DuPage Aurora from 2014 to 2015. In 2015, they joined Kiesque Inc. as a Project Manager for a year.

From 2016 to 2017, Denise Harshbarger worked for CAUSEGEAR, a company in the ethical fashion industry, where they held the role of Partner Development - Servant. Following that, they joined Prairie Path Foot & Ankle Clinic as the Director of Marketing, overseeing marketing strategies and initiatives from 2017 to 2021.

In 2021, Denise Harshbarger joined Weil Foot & Ankle as the Marketing Manager and held that role until 2023. Currently, they are the Director of Marketing at Balance Health, which was formed through a merger with Weil Foot & Ankle.

Denise Harshbarger earned a Graduate Certificate in Public Relations: Corporate Communications from Ball State University in the years 2011 to 2013. Denise also obtained a Certification in Clinical Laboratory Science/Medical Technology/Technologist from Parkview School of Medical Technology in 1996. In addition, Denise Harshbarger received a Bachelor of Science degree in General Science from Penn State University in the years 1993 to 1995. Prior to this, they attended Goshen College from 1991 to 1993, studying Chemistry.