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Adam Zlotnick has a BA in Biology from the University of Virginia and earned his PhD at Purdue University, in 1994, in the lab of Jack Johnson where his thesis topic was the structure and assembly of icosahedral viruses. Dr. Zlotnick went on to be a National Research Council Fellow at the NIH, in the lab of Alasdair Steven, using cryo-EM and solution studies to investigate Hepatitis B virus.

In 1998, Dr Zlotnick took a faculty position at Oklahoma University Health Sciences Center where he was first able to develop approaches to quantitatively observing virus assembly. In 2008, Dr. Zlotnick moved to Indiana University where he is a Professor of Molecular and Cellular Biochemistry. He continues to study the physical chemistry of virus assembly and its structural basis in several systems, particularly HBV. He also works on developing antivirals directed at viral structural proteins. He uses a broad range of structural, physical, and biochemical techniques in his studies.

He co-founded Assembly Pharmaceuticals (now Assembly Biosciences) in 2012 and Door Pharmaceuticals in 2018. He initiated the Gordon Conference series on Physical Virology in 2009. He is a fellow of the American Academy of Microbiology and of the AAAS. He has published more than 130 papers and reviews; he is an inventor on 10 patents and patent applications.