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Rolf Kern has a diverse and extensive work experience spanning several companies. Rolf joined Amunix Pharmaceuticals in 2021 as a Senior Scientist. Prior to this, from 2018 to 2021, they worked at SCIEX as a Staff Scientist in Pharma Technical Marketing. Before that, from 2016 to 2018, they worked at Stemcentrx, Inc. as an Associate Scientist, where they established GLP compliant LCMSMS capabilities and later transitioned to research assays for bioanalysis. Rolf also worked at SCIEX from 2008 to 2016 as a Senior Applications Chemist. In the earlier part of their career, from 1999 to 2008, they worked at Theravance, Inc. as a Research Systems Programmer, where they initially served as an analytical chemist before switching to programming and system analysis and administration. Their professional journey began at Toxscan, Inc. in 1992, where they worked as an Analytical Chemist until 1999.

Rolf Kern attended the University of California, Santa Cruz from 1988 to 1994 where they pursued their studies. However, no specific degree or field of study is mentioned during this period. Later, Rolf Kern obtained a Bachelor's degree in Chemistry from the University of California, Santa Cruz but the exact duration of this degree program is not provided.